Composite
CAPE-AFP

Part:BBa_K4139025:Design

Designed by: Zachary Robinson & Jason Luddu   Group: iGEM22_Lethbridge   (2023-09-27)


CAPE-AFP:


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1052
    Illegal PstI site found at 1223
    Illegal PstI site found at 1394
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
    Illegal NheI site found at 50
    Illegal PstI site found at 1052
    Illegal PstI site found at 1223
    Illegal PstI site found at 1394
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1052
    Illegal PstI site found at 1223
    Illegal PstI site found at 1394
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1052
    Illegal PstI site found at 1223
    Illegal PstI site found at 1394
    Illegal AgeI site found at 529
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Begins with a His-tag to aid with purification followed by three copies of Anti-PbEL04 (CAPE) to increase chances of binding to PbEL04.

Source

Plasmodiophora brassicae

References

Jiang, X., Su, Y., & Wang, M. (2022). A small cysteine-rich protein identified from the Proteome of clubroot pathogen, Plasmodiophora brassicae, induces cell death in nonhost plants and host plants. https://doi.org/10.21203/rs.3.rs-1961445/v1

Lowe, D. G., Ricketts, M., Levinson, A. D., & Goeddelt, D. V. (1988). Chimeric proteins define variable and essential regions of Ha-ras-encoded protein (guanine nucleotide-binding protein/Ha-ras p21/R-ras p23/mammalian transformation). In Proc. Nail. Acad. Sci. USA